Figure 5

Effects of ADRC on the expression of VEGF isoforms in macrophages. Effect of ADRC-CM on the expression of total VEGF-A (a), VEGF-A₁₆₅b (b), F4/80 (c), CD206⁺ cells (d), and TNF-α (e) in peritoneal macrophages from WT mice. Macrophages were pretreated with ADRC-CM or control medium for 24 h. mRNA expression of total VEGF-A, VEGF-A₁₆₅b, and TNF-a was measured by real-time PCR and expressed relative to Gapdh levels (n = 12 in each group). The levels of proteins VEGF-A, VEGF-A₁₆₅b, and TNF-a in the ADRC-CM treated cells were assessed by western blotting analysis (n = 4 in each group). The expression of F4/80 and CD206⁺ cells was analyzed by flow cytometric analysis (n = 4 in each group).