Figure 6 | Scientific Reports

Figure 6

From: In silico analysis and in vivo assessment of a novel epitope-based vaccine candidate against uropathogenic Escherichia coli

Figure 6

Evaluation of the humoral responses in the immunized mice. (A) Mice were vaccinated and the IgG responses and longevity of humoral responses measured. Single asterisks show statistical significance of IgG responses over the control groups from day 45 to 180 post first vaccine inoculation (p < 0.001), and double asterisks show statistical significance of IgG over fusion protein alone (p < 0.05) and control groups (p < 0.001). The results are the average of three independent experiments at serum dilution 1:800. Then, (B) anti-fusion IgG1 & IgG2a and (C) IgA responses were assessed in the mice. Single asterisks show statistical significance of IgG1 & IgG2a and IgA responses over the control groups (p < 0.001), and double asterisk shows statistical significance of IgA over fusion protein alone (p = 0.045). The results are the average of three independent experiments at dilution 1:200 serum. Bars represent mean ± S.D. from 12 mice per groups. Statistical analysis of total IgG, IgG isotypes, and IgA responses was performed using One way ANOVA test.

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