Figure 1

(a) The ESI mass spectrum of α-syn under native conditions shows a monomer charge state distribution ranging from 5+ up to 18+. Low intense dimer peaks are indicated with ‘D’. (b) The CCS heat map shows the populated conformational space per charge state (5+ to 18+) for α-syn monomers. The relative intensity of each CCS profile (vertical columns, per charge state) is normalised separately within a scale from 1: very intense (red) to 0: not present (dark blue), whereas the overall intensities of different charge states relative to each other are apparent in (a). The dashed box shows that for the 8+ state, also indicated in (a), four different conformational families are present.