Figure 3

Performance of single analysis of CD4⁺CD25⁺ T-cells and combined stepwise analysis of CD4⁺CD25⁺ T-cells and CXCL9 for early diagnosis of congenital toxoplasmosis. Scatter plot illustrate the single analysis of CD4⁺CD25⁺ T-cells in infants with congenital toxoplasmosis (TOXO, dark circles, n = 37) and age-matching healthy controls (CTL, white circles, n = 15). The dotted line represents the cut-off previously selected by TG-ROC and ROC curve analysis. The frequencies of TOXO samples (Se) and CTL (Sp) segregated by the cut-offs are displayed in each scatter plot. False-positive and false-negative results are underscored by red circles. Stepwise biomarker analysis was carried out using the frequency of circulating CD4⁺CD25⁺ T-cells percentages for screening and the serum levels of CXCL9 as a second step to segregate infants with congenital toxoplasmosis (TOXO, dark circles, n = 37) from age-matched healthy controls (CTL, white circles, n = 15). The dotted line represents the cut-off previously defined by the ROC curve analysis for single biomarker use. Gray backgrounds display patients selected for the second round of analysis. The proportion of TOXO and CTL across the cut-offs are shown in each scatter plot. The dotted line rectangles show the number of cases accounted in the discriminant analysis as accurate results. The discriminant analysis results, including the single and stepwise biomarker analysis, are demonstrated in the inserted tables below each scatter plot.