Figure 4

Performance of IFN-γ-producing T-cells (∑ IFN-γ⁺ CD4⁺ & CD8⁺) for early diagnosis of congenital toxoplasmosis. TG-ROC was plotted based on the at the y axis versus cut-off at the x axis. The vertical dotted line represents the cut-off with highest accuracy. ROC curves was plotted considering the sensitivity (Se%) and the complement of the specificity (100-Sp%). The performance indices (Cut-off; Area Under the Curve—AUC; Sensitivity (Se); Specificity (Sp); Likelihood Ratio—LR(−)/LR(+) are provided in the figure. Scatter plots illustrate the percentages of IFN-γ⁺ T-cells (∑ IFN-γ⁺ CD4⁺ & CD8⁺) in infants with congenital toxoplasmosis (TOXO, dark circles, n = 49) and age-matching healthy controls (CTL, white circles, n = 10). The dotted line represents the cut-off previously selected by TG-ROC and ROC curve analysis. The frequencies of TOXO samples (Se) and CTL (Sp) segregated by the cut-offs are displayed in the scatter plot.