Figure 5

Performance of intracellular cytokines produced by T-cells for the early prognosis of ocular congenital toxoplasmosis. The selected biomarkers, IL-5⁺CD4⁺ T-cells and IFN-γ⁺NK-cells, were evaluated for their performance as novel laboratorial parameters for early prognosis of ocular congenital T. gondii infection. The performance of IL-5⁺CD4⁺ T-cells was tested to discriminate infants with congenital toxoplasmosis with (L) from those without (NL) retinochoroidal lesions. The frequency of IFN-γ⁺NK-cells was tested for its ability to segregate infants with active (AL) or cicatricial (CL) retinochoroidal lesions. TG-ROC was built considering the sensitivity (Se) and specificity (Sp) at the y axis versus cut-off at the x axis. The vertical dotted line shows the cut-off with highest accuracy. ROC curves were plotted considering the sensitivity (Se%) and the complement of the specificity (100-Sp%). The performance indices (Cut-off; Area Under the Curve—AUC; Sensitivity (Se); Specificity (Sp); Likelihood Ratio—LR(−)/LR(+) are provided in the figure. Scatter plots illustrate the percentages of IL-5⁺CD4⁺ T-cells in infants with (L, dark circles, n = 41) or without (NL, white circles, n = 10) retinochoroidal lesions as well as the percentage of IFN-γ⁺NK-cells in infants with active (AL, white circles, n = 14) or cicatricial retinochoroidal lesion (CL, dark circles, n = 12). The dotted line displays the cut-offs selected by TG-ROC and ROC curve analysis. The frequencies of infants above and below the cut-offs are displayed in each scatter plot.