Figure 5
From: Biochemical evidence of both copper chelation and oxygenase activity at the histidine brace
Oxidation of ascorbic acid in combination with different scavengers of oxygen species. (a) 1 µM TaAA9A, (b) 1 µM Bim1, and (c) buffer was equilibrated with 0.5 µM CuCl2 overnight and tested together with BCA (red), EDTA (blue), 10 nM superoxide dismutase and 1 nM catalase (green), 10 nM superoxide dismutase (purple), 1 nM catalase (orange) and without scavengers (black). 20 mM MES buffer pH 6.6 and 100 µM ascorbic acid, incubated at 26 °C. The absorption was read every minute with intermittent shaking. (d) Initial turnover frequency (s-1) of ascorbate oxidation determined by an approximate linear fit to the progress curve. The values for CopC and negative controls with buffer only are included in the table.
