Figure 7
From: Biochemical evidence of both copper chelation and oxygenase activity at the histidine brace
UV and X-band EPR absorption spectroscopy after ascorbate treatments. (a) Progress curves for oxidation of 100 µM ascorbate in 20 mM MES pH 6.6 at 26 °C with 1 µM PfCopC (blue), PfCopCE27A (black), PfCopCH3F (blue dots), PfCopCA2P (black dashes), Bim1 (green), TaAA9A (purple) loaded with 0.5 µM Cu(II). (b) EPR spectra of 500 µM PfCopC (blue), 250 µM PfCopCE27A (black), 1.5 mM TaAA9A (purple) in the presence of 10 mM ascorbic acid.
