Table 2 Experimental procedures with timeline (per trial).
Timeline | Number of chickens | |||||
|---|---|---|---|---|---|---|
Daily probiotic colonization | Enteric bacterial challenge | Fecal sample collection | Euthanization | Cecum sample harvest | Live | |
Day0 | – | – | 60 (1/chicken) | – | – | 60 |
Day1–Day7 | 40 (B, C)a | – | – | – | – | 60 |
Day8 | – | 60 (A, B, C)a | 60 (1/chicken) | – | – | 60 |
Day14 | – | – | 60 (1/chicken) | 18 (6/group) | 18 (6/group) | 42 |
Day21 | – | – | 42 (1/chicken) | 18 (6/group) | 18 (6/group) | 24 |
Day28 | – | – | 24 (1/chicken) | 24 (8/group) | 24 (8/group) | 0 |
