Figure 4
From: Fast and antibiotic free genome integration into Escherichia coli chromosome
Colony PCR after GFP genome integration according to pAIO protocol. (A) Expected PCR product (1960 bp) in case of successful GFP integration. (B) Expected PCR product (868 bp) of empty genomic locus in case of failed genome integration. (C) Colony screening of 16 single BL21(DE3) < GFP > colonies. (D) Colony screening of 16 single HMS174(DE3) < GFP > colonies whereas colonies 3, 4, 8, 9, 13 were confirmed as positive integrants. (L…NEB 1 kb plus ladder).
