Table 2 Primer names and sequences.
From: Fast and antibiotic free genome integration into Escherichia coli chromosome
TN7_HO1 | AGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATA GTAGTAGGTTGAGGCCGTTG |
TN7_HO2 | CAGCCGCGTAACCTGGCAAAATCGGTTACGGTTGAGTAATAAATGGATGC CGGATATAGTTCCTCCTTTCAG |
EcoRI_pROCOLI_sense | GGAGGAATTCACCATGGTACCCGG |
NheI_pROCOLI_as | CTCCTGCTAGCCCAAAAAAACGGG |
FRT_cm_sense | AGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAtagggataacagggtaatTAAGCAGAAGGCCATCCTGAC |
FRT_cm_as | CAGCCGCGTAACCTGGCAAAATCGGTTACGGTTGAGtaaTAAATGGATGCGCGAACGTGGCGAGAAAGG |
TN7/2_GFP | CAGCCGCGTAACCTGGCAAAATCGGTTACGGTTGAGTAATAAATGGATGCATTTAGAGCTTGACGGGG |
SceIRS_sense | tagggataacagggtaatCGTAGAGGATCTGCTCATG |
SceIRS_as | TCTCACCTACCAAACAATGC |
TN7/1_ext | ACCGGCGCAGGGAAGG |
TN7/2_ext | TGGCGCTAATTGATGCCG |
BglII_SceI_sense | atcatcagatctGATGCGTCCGGCGTAGAGG |
BglII_SceI_as | atcatcagatctTTCGCGGCCGCTGTATTTAG |
