Figure 3
From: Progressive optic atrophy in a retinal ganglion cell-specific mouse model of complex I deficiency
Assessment of NDUFS4 protein expression in mouse optic nerves. (A) Representative Western blot comparing the expression of NDUFS4 and actin in optic nerve lysates from ndufs4loxP/loxP and Vglut2-Cre; ndufs4loxP/loxP mice at P30. Total protein content of lysate loaded into each lane is indicated below, and the presence or absence of the Vglut2-Cre transgene is indicated above each lane. The graphs to the right depict NDUFS4 and actin band intensities plotted as a function of total protein loaded, demonstrating that the intensities are within a linear range for both proteins. (B) Quantification of NDUFS4 protein content in Vglut2-Cre;ndufs4loxP/loxP optic nerve lysates relative to ndufs4loxP/loxP littermates. NDUFS4 band intensity was normalized to actin band intensity for each sample, with 12.5 µg of total protein loaded per sample. n = 6 mice per genotype. Data are presented as mean ± SEM. See Supplementary Fig. S2 online for the uncropped blot from panel A.
