Figure 2
From: Tax1BP1 limits hepatic inflammation and reduces experimental hepatocarcinogenesis
Increased number of leukocytes and increased inflammatory responses in Tax1BP1−/− mice. Number of all leukocytes (A) and different subpopulations of leukocytes (B) were determined by FACS from livers of Tax1BP1+/+ and Tax1BP1−/− mice, respectively. mRNA of TNFα, IL-6 and IL1β was determined by quantitative PCR in Kupffer cells extracted from two livers of Tax1BP1+/+ and Tax1BP1−/− mice ± stimulation with LPS 300 ng/ml for 6 h. Relative expression of mRNA was normalized to unstimulated Tax1BP1+/+ mice. Representative results of two independent experiments are shown (C–E). (*P < 0.05; **P < 0.01; GITR glucocorticoid-induced tumor necrosis factor receptor related protein, NK cells natural killer cells, MDM monocyte-derived macrophages, KC Kupffer cells, DC dentritic cell).
