Figure 1
From: Glycosylation of Trypanosoma cruzi TcI antigen reveals recognition by chagasic sera
T. cruzi TSSA recombinant proteins produced in L. tarentolae expression system. (a) gTSSA-I protein sequence (based on GenBank GU059925) and (b) gTSSA-II/V/VI protein sequence (based on GenBank GU075675): histidine tag (blue), SUMO sequence (green), linker sequences (black) and TSSA sequences (red). Dots: Residues predicted to be O-glycosylated. Starred: Ser residue present in native TSSA-II/V/VI but absent in native TSSA-I. Underlined: lineage-specific polymorphic residues. (c) Predicted likelihood scores for O-glycosylation of TSSA-I sequence range between 0 (least)—1 (highest); scores below 0.5 not listed. (d) Coomassie blue stained gel of purified gTSSA-I.
