Table 1 Samples and corresponding DNA extraction strategy.

From: Non-specific amplification of human DNA is a major challenge for 16S rRNA gene sequence analysis

Sample

DNA extraction strategy

Breast: tumour and normal

Molzym Ultradeep Microbiome (Molzym, Bremen, Germany)

Oesophageal biopsies

AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany) with modifications10

Skin Swab samples

QIAamp UCP Pathogen Mini Kit (Qiagen, Hilden, Germany)

Stool samples

Repeated bead beating method as previously described, with modifications11, 12