Table 1 Samples and corresponding DNA extraction strategy.
From: Non-specific amplification of human DNA is a major challenge for 16S rRNA gene sequence analysis
Sample | DNA extraction strategy |
|---|---|
Breast: tumour and normal | Molzym Ultradeep Microbiome (Molzym, Bremen, Germany) |
Oesophageal biopsies | AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany) with modifications10 |
Skin Swab samples | QIAamp UCP Pathogen Mini Kit (Qiagen, Hilden, Germany) |
Stool samples | Repeated bead beating method as previously described, with modifications11, 12 |