Figure 2 | Scientific Reports

Figure 2

From: Saturation transfer difference NMR on the integral trimeric membrane transport protein GltPh determines cooperative substrate binding

Figure 2

Selected spectral region of the reference 1H NMR (top) and corresponding STD NMR spectrum of GltPh incubated with 0.5 mM L-glutamate in the presence (middle) and absence (bottom) of 100 mM Na+ ions; (a) detergent solubilised GltPh (~ 20 μM) and (b) GltPh (~ 20 μM) reconstituted into proteoliposomes (800 MHz, 5 °C, 2 s saturation time). Full spectra can be found in the Supporting Information.

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