Figure 4 | Scientific Reports

Figure 4

From: Saturation transfer difference NMR on the integral trimeric membrane transport protein GltPh determines cooperative substrate binding

Figure 4

Competition binding of L-aspartate in the presence of excess L-glutamate (1.5 mM) in GltPh. (Top) Normalised STD effect (%) as a function of L-aspartate concentration for GltPh in detergent micelles (left) and reconstituted proteoliposomes (right). The solid line represents the best fit according to Eq. (3). (Bottom) Normalised data fitted to a standard Hill equation (Eq. 4), resulting in Hill coefficients of 1.06 and 0.69 in detergent micelles (left), and reconstituted proteoliposomes (right) respectively.

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