Figure 4 | Scientific Reports

Figure 4

From: Synthesis and structure of a new thiazoline-based palladium(II) complex that promotes cytotoxicity and apoptosis of human promyelocytic leukemia HL-60 cells

Figure 4The alternative text for this image may have been generated using AI.

Effects of chemotherapeutics on caspases activation in HL-60 cells. Cells were treated with 11.3 µM cisplatin, 20.7 µM PyTT, 20.7 µM PdPyTT, or the vehicle (DMSO, control) for 24 h. Caspase-3 (A) and -9 (B) activation was measured by flow cytometry by means of CaspGLOW fluorescein active caspase-3 staining kit and Caspase-9 staining kit (Red-LEHD-FMK), respectively. Analysis was restricted to live cells that were detected by using the vital dye Hoechst 33258. Representative cytograms of each experimental condition are shown for both caspases. (C and D) Histograms show percentages of each cell population. Values represent means ± S.D. of 6 independent experiments. *P < 0.05 compared to control values.

Back to article page