Figure 5 | Scientific Reports

Figure 5

From: Synthesis and structure of a new thiazoline-based palladium(II) complex that promotes cytotoxicity and apoptosis of human promyelocytic leukemia HL-60 cells

Figure 5The alternative text for this image may have been generated using AI.

Effects of chemotherapeutics on the intracellular production of superoxide anion and reactive oxygen species (ROS) in HL-60 cells. Cells were treated with 11.3 µM cisplatin, 20.7 µM PyTT, 20.7 µM PdPyTT, or the vehicle (DMSO, control) for 24 h. The production of mitochondrial superoxide anion (A) and intracellular ROS (B) were determined by flow cytometry by means of MitoSOX and DCFH, respectively. Analysis was restricted to live cells that were detected by using the vital dye Hoechst 33,258. Representative cytograms of each experimental condition are shown. (C) Histograms show percentages of each cell population. Values represent means ± S.D. of 6 independent experiments. *P < 0.05 compared to control values.

Back to article page