Figure 6

Chemotherapeutics disturbed cell cycle profile of HL-60 cells. Cells were treated with 11.3 µM cisplatin, 20.7 µM PyTT, 20.7 µM PdPyTT, or the vehicle (DMSO, control) for 24 h. Cell cycle distribution was determined by flow cytometry analysis using ethanol-fixed, propidium iodide-stained cells. Data from the cell cycle distribution were summarized and presented as percentage of cells. Cells with hypodiploid DNA content (sub-G1/G0 population) denote an increase in DNA fragmentation. Histograms show percentages of apoptotic cells. Values represent means ± S.D. of 6 independent experiments. *P < 0.05 compared to its corresponding control values.