Figure 7 | Scientific Reports

Figure 7

From: Synthesis and structure of a new thiazoline-based palladium(II) complex that promotes cytotoxicity and apoptosis of human promyelocytic leukemia HL-60 cells

Figure 7The alternative text for this image may have been generated using AI.

Effects of chemotherapeutics on DNA oxidative damage in HL-60 cells. Cells were treated with 11.3 µM cisplatin, 20.7 µM PyTT, 20.7 µM PdPyTT, or the vehicle (DMSO, control) for 24 h. (A) DNA oxidative damage was determined by flow cytometry in fixed cells labelled with anti-8-OHdG antibody and F(ab')2-goat anti-mouse IgG (H + L) secondary antibody, Alexa Fluor 633. Analysis was restricted to nucleated cells that were distinguished by staining cells with PI. Representative cytograms of each experimental condition are shown. (B) Histograms show percentages of each cell population. Values represent means ± S.D. of 6 independent experiments. *P < 0.05 compared to control values.

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