Figure 8 | Scientific Reports

Figure 8

From: Synthesis and structure of a new thiazoline-based palladium(II) complex that promotes cytotoxicity and apoptosis of human promyelocytic leukemia HL-60 cells

Figure 8The alternative text for this image may have been generated using AI.

The complex PdPyTT lacks DNA intercalating capacity. (A) DNA intercalation was assessed by means of a DNA unwinding assay kit, as explained in Materials and methods. Lane 1: supercoiled pBR322; lane 2: supercoiled pBR322 + wheat germ Topoisomerase I; lane 3: supercoiled pBR322 + wheat germ Topoisomerase I + 20.7 µM PdPyTT; lane 4: supercoiled pBR322 + 20.7 µM PdPyTT; lane 5: relaxed pBR322; lane 6: relaxed pBR322 + wheat germ Topoisomerase I. If a given compound were an intercalating agent, it would unwind the supercoiled DNA or displace topoisomerase I from the groove, thus producing DNA mobility shifts which is not the case for the complex PdPyTT. (B) Western blot analysis of whole-cell lysates to detect p53 expression in HL-60 cell line 24 h after treatment with 11.3 µM cisplatin, 20.7 µM PyTT, 20.7 µM PdPyTT, or the vehicle (DMSO, control). GAPDH levels were used as loading controls. For the shake of clarity, gels/blots were cropped and merged in a single image as they were obtained from different parts of the same gel/membrane. Full-length gels and blots are presented in Supplementary Figures S10 and S11, respectively. (C) Histograms represent p53 protein levels normalized to GAPDH content and are presented as fold change. Data are presented as mean ± S.D. of 3 independent experiments. *P < 0.05 compared to control values.

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