Figure 3
Objective measurement of Gleason 4 burden shows a discrepancy between visual measurement and the digital measurement for the index block. (A) Visual %G4 for the index block 30 patients shown in yellow overlaid with digital %G4 in blue. Patients separated by original Gleason grade grouping; 3 + 4 or 4 + 3, and organized by visual %G4. A threshold of 50% G4 for clinical significance is shown as a red dashed line. Patient number on the x-axis highlighted in bold and underlined if the objective measurement of their %G4 would cause reclassification. (B) Bland–Altman plot representing the difference in measurement in the y-axis as visual %G4 − digital %G4. The x-axis represents the mean %G4 measurement of both techniques as (visual %G4 + digital %G4)/2. The bold black line represents complete agreement at 0. The purple dashed line corresponds to the bias at 14.36; the dotted purple line corresponds to the bias confidence interval (9.78–18.94). Dash and dotted blue lines correspond to the upper and lower limit of agreement and confidence intervals are plotted with dotted blue lines. Upper limit of agreement: 38.40 (30.49–46.32), lower limit of agreement: − 9.67 (− 17.59 to − 1.76). The regression line is plotted as a continuous blue line.
