Figure 1
From: Species-specific differences in synaptic transmission and plasticity
Macroanatomy of the Etruscan shrew hippocampus is similar to mice. Sagittal sections stained for DAPI showing the hippocampus of (a) shrew and (b) mice indicating the different subregions of the hippocampus—dentate gyrus (DG), CA1, CA3 and the pyramidal (Py), Stratum Radiatum (Sr) and Stratum Lucidum (SL) layers. (c) Relative cell densities in different hippocampal subregions do not differ between shrews (green) and mice (purple), indicating conserved cytoarchitectonic layout (p-values based on Mann–Whitney two tailed test.). (d,f) Saggital section of an Etruscan shrew hippocampus labelled for calbindin (CB, green) and DAPI (blue) indicating the different hippocampal subregions—CA1, CA2, CA3, dentate gyrus (DG) and mossy fibers (mf). (e) Mossy fibers (mf) visualized by the presence of synaptic zinc (brown) in a sagittal section of an Etruscan shrew brain, and CA2 labelled by the presence of the protein PCP4 (yellow) show that the mossy fibers are present in CA3 and terminate at the CA2 region. (g) Sagittal section of a mouse, marked same as (f). (h) Relative convergence ratios between mossy fiber intensities and cell density reveal a higher mossy fiber to CA3 pyramidal cell convergence factor in shrews than in mice.
