Figure 3
Activation through CD300e does not interfere with the intracellular trafficking of HLA-DR. (a) Kinetic of internalization and recycling of HLA-DR in monocytes. The amount of HLA-DR remaining or recycling back to the cell surface in untreated monocytes and in monocytes exposed to control IgG or anti-CD300e was determined by flow cytometry. For each sample, MFI values are expressed as percentage relative to T0 or to time 120 min (set as 100%). Data are shown as mean ± SEM of 5 independent experiments. (b) HLA-DR total cell content evaluated by western blot in monocytes seeded on uncoated, coated with isotypic IgG or with anti-CD300e for 24 h. Blot refers to a representative of 4 independent experiments. Quantification of HLA-DR was performed by densitometry and normalized to vinculin (mean ± SEM). Significance was determined by Student’s t-test. *p ≤ 0.05.
