Figure 6 | Scientific Reports

Figure 6

From: Perfusion reduces bispecific antibody aggregation via mitigating mitochondrial dysfunction-induced glutathione oxidation and ER stress in CHO cells

Figure 6

Analysis of ER stress in fed-batch and perfusion processes. Cell pellets were collected on the indicated days from a fed-batch and a perfusion bioreactor to determine protein expression of ATF-6, CHO and BiP using capillary western blot analysis. FKBP12 was used as a loading control. (A) Representative western blot images for ATF-6 (~ 100 kDa), CHOP (~ 27 kDa), BiP (~ 70 kDa) and FKBP12 (~ 12 kDa) are shown. (B–D) Densitometric analysis of western blot. Data are expressed as target protein AUC/loading control AUC.

Back to article page