Figure 6
Workflow schematic. General schematic of pipeline input and output. Reconstructions are not to scale. For detection and 2D segmentation, either Pomegranate (red lines) or an external algorithm (blue lines) can be used. For Pomegranate 2D segmentation, a brightfield Z-stack is required as whole-cell input image, a fluorescence Z-stack is required as nuclear input image. After reconstruction and 3D segmentation, any desired signal that was co-recorded (“Tagged protein”) can be quantified.
