Figure 6 | Scientific Reports

Figure 6

From: Three-dimensional data capture and analysis of intact eye lenses evidences emmetropia-associated changes in epithelial cell organization

Figure 6

Analysis pipeline and LEC nuclei definition. (A) Processing pipeline, from sample imaging to 3D map generation. A z-stack is captured for each lens sample and reconstructed prior to the detection of LEC nuclei. Density analysis of ROIs can be performed as shown in Fig. 8A, and 3D coordinate maps of detected nuclei are produced following the user-definition of an equatorial plane and the subsequent geometrical definition of the anterior pole. (B) LEC nucleus detection is based on user-defined circularity and thresholding values. Image quality appropriate thresholding are required for successful watershed segmentation of partially overlapping nuclei.

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