Figure 8

ROI analysis and LEC nuclei global coordinate assignment. (A) ROI analysis output: the maximum intensity guide image with the color-annotated grid and segmented nuclei is produced in parallel to the LEC nuclear density graph. (B) ROI and global coordinate assignment output: the segmented nuclei from the ROI grid are reconstructed in 3D according to the image metadata. All detected nuclei, the user-defined points (p1, p2, p3), the near-equatorial circle plane and the perpendicular vector are also reconstructed in 3D.