Figure 2 | Scientific Reports

Figure 2

From: Sofosbuvir terminated RNA is more resistant to SARS-CoV-2 proofreader than RNA terminated by Remdesivir

Figure 2

Polymerase reactions with SOF-TP, UTP or UTP + RDV-TP to produce RNA extension products. The sequence of the RNA template-loop-primer used for these polymerase extension reactions is shown at the top of the figure. (a) MALDI-TOF mass spectrum of the unextended RNA. Polymerase extension reactions were performed by incubating (b) SOF-TP, (c) UTP and (d) UTP + RDV-TP with pre-assembled SARS-CoV-2 polymerase (nsp12, nsp7 and nsp8) and the indicated RNA template-loop-primer, followed by detection of reaction products by MALDI-TOF MS. The accuracy for m/z determination is approximately ± 10 Da.

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