Figure 5

Device throughput can be increased by running with a higher cell concentration. Transfection efficiency is presented for resting T-cell concentrations ranging from 2 to 50 million/ml, and mCherry-encoding mRNA concentrations of 20 μg/ml and 50 μg/ml. High transfection efficiency (70–85%) is maintained at cell concentrations up to 50 million/ml. At the highest cell concentration tested, the device processed approximately 20 million cells/min. The data for 50 million cells/ml was only run with the 50 μg/ml mRNA concentration. These data are an average of replicates from three independent, healthy donors (n = 3). The same three donors were used for experiments run at 2, 10, and 20 million cells/ml, and a new set of three donors was used for experiments run at 50 million cells/ml. Error bars represent standard error of the mean. This figure was generated using GraphPad Prism software.