Figure 6 | Scientific Reports

Figure 6

From: High-throughput continuous-flow microfluidic electroporation of mRNA into primary human T cells for applications in cellular therapy manufacturing

Figure 6The alternative text for this image may have been generated using AI.

Long-term effects of continuous-flow microfluidic electrotransfection on T-cell viability and expansion rate. T cells were transfected with mRNA on Day 0. (A) Viability of activated, expanding T cells over a period spanning 13 days normalized to the pre-transfection viability on Day 0. After a short-term drop in viability by 5–13% 24 h after transfection, viability remained stable and similar to that of control cells that were not transfected or introduced into our device. (B) Population doubling times for transfected cells were nearly indistinguishable from control cells. (C) Comparison of mCherry expression over time post transfection between activated T cells and resting T cells. (D) Comparison of the total number of mCherry-expressing cells over time post transfection between activated T cells and resting T cells. The total cell number is normalized to the total number of cells on day 1 (24 h after transfection). All data presented are an average of replicates from three independent, healthy donors (n = 3) and error bars represent standard error of the mean. Some error bars are too small to be visible. This figure was generated using GraphPad Prism software.

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