Figure 7 | Scientific Reports

Figure 7

From: High-throughput continuous-flow microfluidic electroporation of mRNA into primary human T cells for applications in cellular therapy manufacturing

Figure 7The alternative text for this image may have been generated using AI.

Our device is consistent and effective when used for transfection of clinically-relevant numbers of cells (approximately 200 million–500 million cells per donor). (A) Representative data from one primary human T-cell donor. All of the T cells from one buffy coat donor (approximately 500 million cells total) were activated, resuspended in BTXpress electroporation media at 50 million cells/ml, and then transfected with mCherry-encoding mRNA (50 μg/ml) continuously in a single microfluidic electroporation channel over a time span of approximately 20 min. Samples taken at approximately 4-min intervals show that transfection efficiency and viability (measured 24 h post-transfection) are consistent throughout processing. (B) T cells were isolated from buffy coat samples from three independent, healthy donors and activated. Each donor produced approximately 200 million–500 million T cells. The entirety of each donor sample was then transfected with mCherry mRNA. Average transfection efficiency and viability ratio across the three donors is shown (n = 3), where error bars represent standard error of the mean. This figure was generated using GraphPad Prism software.

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