Table 1 Primer nucleotide sequences and combinations used to amplify NPM1 splice variants with either wild-type or mutation A containing alleles.
From: Preferential transcription of the mutated allele in NPM1 mutated acute myeloid leukaemia
Target | Sense strand 5′-3′ | Antisense strand 5′-3′ |
|---|---|---|
NPM1.1 wild-type | CAACACCAAGATCAAAAGG | CCTCCACTGCCAGAGA |
NPM1.1 mutated | CAACACCAAGATCAAAAGG | CTTCCTCCACTGCCAGACAGA* |
NPM1.2 wild-type | GAAGAAAGGACAAGAATCC | CCTCCACTGCCAGAGA |
NPM1.2 mutated | GAAGAAAGGACAAGAATCC | CTTCCTCCACTGCCAGACAGA* |
NPM1.3 | CAACACCAAGATCAAAAGG | CTGTTCAATGCGCTTTTTC |
