Figure 1

Stimulation of gliadin-reactive T cells by B-LCL with different genotypes. (A) Shows a representative experiment of the dose curve IFN-γ response by a DQ2.5-glia-α1,2-specific TCL obtained from celiac gut mucosa. Intestinal cells were stimulated by B-LCL#5 (from a celiac DR1/DR3), B-LCL#23 (from a celiac DR5/DR7 ), B-LCL#29 (from a control DR5/DR7). The APCs were pulsed with DQ2.5-glia-α1,2 peptide at different concentrations (0.01, 0.1, 1, 10 µM). (B) Shows the average of IFN-γ produced by a DQ2.5-glia-α1,2 -specific TCL at the sub-optimal DQ2.5-glia-α1,2 peptide concentration (1 µM). DR1/DR3 B-LCLs histogram represents the result of N = 5 experiments performed using as APCs celiac B-LCL#5 and B-LCL#6; DR5/DR7 B-LCLs histogram shows the result of N = 9 experiments performed using as APCs celiac B-LCL#23, #25, #26 and #27; DR5/DR7 CTR B-LCLs histogram represents the IFN-γ mean value of N = 9 experiments performed with B-LCL#29, #30, #31, #35 from healthy subjects. Table in panel B displays results of statistical analysis performed using the unpaired Student's t-test to compare B-LCLs with different genotypes, as indicated. *p < 0.05 was considered statistically significant.