Figure 2 | Scientific Reports

Figure 2

From: Development of an antibody-dependent cellular cytotoxicity reporter assay for measuring anti-Middle East Respiratory Syndrome antibody bioactivity

Figure 2The alternative text for this image may have been generated using AI.

The establishment of stable 293T/MERS cell line. (A) 293T cells were transiently transfected with pcDNA4-MERS-S plasmid and were used as the target cell 24 h after the transfection (2.5 × 104 cells/well), while the Jurkat/NFAT-luc/FcγRIIIa cells were used as effector cells (5 × 104 cells/well). The AF-h2E6 antibodies were diluted and added to the cell mixture at a final concentration as indicated. The cultures were incubated at 37 °C with 5% CO2 for 24 h before the RLU was read. (B) A representative result of the antigen-positive clone screened by the flow cytometry. (C) 293T/MERS clone was selected by the ADCC reporter gene assay.

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