Figure 1

NOSs inhibition leads to late embryonic hind limb developmental defect. (A) Pregnant rats were consecutive administration L-NAME that started at embryonic day 13.5 (E13.5) by peritoneal injection, the embryos were harvest at different time point and multiple phenotypes were check. L-NAME administration induced embryonic hind limb developmental defects, which account for 20%. Approximately 5% embryos displayed fore limb hemorrhage. In addition, no more than 2%, shown cerebral defect, or embryonic lethality. (B) Whole mount images to show hind limb developmental defect after inhibition of NO production. Images obtained from Nikon SMZ745 microscope, Scale bar: 1Â cm.