Figure 3

Suppression of No production suppresses vascular endothelial cells development and maturation. (A) IHC staining against CD31 antibody to visualize the vascular endothelial cells development at E18.5. Relative expression of CD31 according to IOD and small vessel numbers quantified in (B) (n = 4 mice per group, unpaired t-test) and (C) (n = 4 mice per group, unpaired t-test). The vessel development at E20.5 displayed at (D,E) (n = 4 mice per group, unpaired t-test) and (F) (n = 4 mice per group, unpaired t-test). Scale bar: 60 μm. Data presented as means ± SEM, *P < 0.05.