Figure 5 | Scientific Reports

Figure 5

From: Inhibition of eNOS by L-NAME resulting in rat hind limb developmental defects through PFKFB3 mediated angiogenetic pathway

Figure 5

Suppression of NO production decreases the expression of PFKFB3 in vivo and in vitro. (A) HUVEC treated with L-NAME (1 μM) for 30 h and Real Time PCR performed to detect the expression of PFKFB3 (n = 6 independent experiments, paired t-test). (B) HUVEC treated with different dose of L-NAME (0.5 μM, 1.0 μM and 5.0 μM) for 48 h and western blot performed to investigate PFKFB3 protein level. PFKFB3 protein levels in (B) quantified in (C) (n = 4 independent experiments, paired t-test). (D) IF staining against with PFKFB3 and CD31 antibodies at E18.5, (E) IF staining against with PFKFB3 and CD31 antibodies at E20.5. Scale bar: 15 μm. Data presented as means ± SEM, *P < 0.05.

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