Figure 2
From: Rab35 and its effectors promote formation of tunneling nanotubes in neuronal cells
ACAP2 promotes the formation of functional TNTs: (a) Confocal images of cells transfected with GFP, GFP-ACAP2-WT. Arrows indicate TNTs. (b) Bar graphs representing the relative percentage of TNT-connected cells described in (a) (GFP = 100%, GFP-ACAP2 = 124.4 ± 0.1%). (c) Bar graph showing the relative percentage of acceptor cells containing DiD-labelled vesicles from the co-cultures, where donor cells were transfected with GFP, GFP-ACAP2 (GFP = 100%, GFP-ACAP2 = 114.1 ± 6.9%). All graphs from at least three independent experiments and show mean ± SEM. (ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001; by unpaired Student’s t-test). All bar graphs were analysed and represented using Graph Pad Prism version 7. Scale bars 10 µm.
