Figure 3
From: Rab35 and its effectors promote formation of tunneling nanotubes in neuronal cells
Inactivation of ARF6 leads to an increase in TNTs: (a) Confocal images of cells transfected with GFP, GFP-ARF6-WT, GFP-ARF6-Q67L, GFP-ARF6-T27N. Arrows indicate TNTs. (b) Bar graphs representing the relative percentage of TNT-connected cells described in (a) (GFP = 100%, GFP-ARF6-WT = 69.4 ± 4.6%, GFP-ARF6-Q67L = 69.2 ± 3.7%, GFP-ARF6-T27N = 142.2 ± 4.7%). (c) Bar graph showing the relative percentage of acceptor cells containing DiD-labelled vesicles from the co-cultures, where donor cells were transfected with GFP, GFP-ARF6-WT, GFP-ARF6-Q67L, GFP-ARF6-T27N (GFP = 100%, GFP-ARF6-WT = 100.2 ± 2.2%, GFP-ARF6-Q67L = 84.1 ± 3.5%, GFP-ARF6-T27N = 125.8 ± 6.1%). All graphs from at least three independent experiments and show mean ± SEM. (ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001; by one- way ANOVA with Tukey’s multiple comparison post-test). All bar graphs were analysed and represented using Graph Pad Prism version 7. Scale bars 10 µm.
