Figure 4
The impairment of viability and growth of C. albicans by Lcr35 is contact independent. The proximity-dependent mechanism of Lcr35 was tested using cell culture inserts (a). The microorganisms were cultivated with 0.4 µm-pore-size membrane to separate cells with Lcr35 in the bottom chamber and Candida albicans in the top chamber. Anti-Candida effect of Lcr35 cell free supernatant was evaluated on C. albicans viability at 37 °C over 48 h (a). The impact of cell free supernatant on C. albicans growth was evaluated during 24 h after inoculation in medium with Lcr35 supernatant (b) or Lcr35 + STS cell free supernatant (c). The C. albicans growth was followed by OD600 nm every 15 min. C. albicans control was inoculated in MRS (b) or MRS with 1 g/l STS (c). N = 3, *p < 0.05, **p < 0.01, ***p < 0.001 (one-way ANOVA test and Bonferroni correction).
