Figure 4

(A) Sessile contact angles of differently conditioned bud secretion droplets on various substrates, as well as of water and oil droplets on a native alive bud. Different small letters indicate statistical differences between substrates for the same conditioning, capital letters between conditioning for the same substrate (two-way analysis of variance, balanced design, followed by all pairwise multiple comparison procedures Tukey test, p = 0.05; substrate: F_3,199 = 653.3, p < 0.001; condition: F_3,199 = 4.3, p = 0.002; substrate and secretion conditioning did not exert interactive effects: substrate × condition: F_3,199 = 1.8, p = 0.057). The asterisk corresponds to the statistical difference between the water and oil contact angles on bud: t test, t = − 6.3, p ≤ 0.001 (n = 10 per fluid). (B) Images of the bud secretion droplets on different substrates and a bud with an abaxially adhering droplet of water. (C) Relative change of the bud secretion mass on the normal glass after 7-day storage at different conditions. No significant differences in mass before and after storage for the same condition were detected (Table S3). Different small letters indicate statistic differences between treatments (Kruskal–Wallis one-way ANOVA on ranks, H_3,98 = 80.1, p ≤ 0.001, followed by all pairwise multiple comparison procedures Tukey Test, p < 0.05).