Figure 5 | Scientific Reports

Figure 5

From: Mapping the native interaction surfaces of PREP1 with PBX1 by cross-linking mass-spectrometry and mutagenesis

Figure 5

Proximity Ligation Assay of the interaction of PBX1 with wild-type and mutant PREP1. The PREP1-PBX1 complex is visualized by PLA in A549 cells transfected with different GFP-PREP1 constructs (indicated) and using anti-PBX1 and anti PREP1 antibodies (see Methods), which generates red foci when the two proteins are in close proximity. Panel A shows the number of PLA foci counted in the different cells transfected with the indicated constructs. The data are the average of two independent experiments. Nuclear foci are represented in the histogram in dark grey, cytoplasmic foci in light grey. The number of cells counted is reported at the bottom of each histogram bar. Panels B-G show representative confocal images of the PLA experiment in A549 cells transfected with wild-type or mutant PREP1 as indicated. Panel F, shows cells in which only GFP was overexpressed to define the background signal. In panel G, PREP1 ΔHD nuclear foci are comparable to those of the PREP1 wild-type.

Back to article page