Figure 2
From: Structural basis of peroxidase catalytic cycle of human Prdx6
Determination of Peroxidase Activity of Prdx6 Using HRP competitive assay. The plot of percentage inhibition of HRP oxidation by hydrogen peroxide in the presence of reduced and oxidised human Prdx6 as an indicative of the peroxidase activity of Prdx6, such that, higher the percentage inhibition of HRP oxidation by another peroxidase, greater is the catalytic efficiency of that peroxidase. Inset. HRP alone (10 μM, black), HRP (10 μM) followed by addition of 5 μM hydrogen peroxide (red), HRP (10 μM) followed by hydrogen peroxide (5 μM) mixed with 5 μM reduced Prdx6 (blue) or 5 μM oxidised Prdx6 (green); the spectrum was scanned 10 s later.
