Table 1 Quantification of Total Thiol Content in the Prdx6 Samples Using Absorption Spectroscopy and DTNB Assay.

From: Structural basis of peroxidase catalytic cycle of human Prdx6

 

Absorption spectroscopy [Protein]a ([expected thiol]b) (μM)

DTNB assay (Total thiol) (μM)

Reduced Prdx6

20 (40)

37 ± 1.2

Oxidised Prdx6

20 (40)

0.5 ± 0.2

  1. aAs measured photometrically at 280 nm using the extinction coefficient (ε280 = 2.24 × 105 M−1 cm−1).
  2. bAs calculated by multiplying the protein concentration with no. of Cys residues in the protein (Human Prdx6 has 2 Cys residue).
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