Figure 3
From: Cadherin repeat 5 mutation associated with Bt resistance in a field-derived strain of pink bollworm
Toxic effects of Cry1Ac on transformed Hi5 cells producing PgCad1 fusion proteins. Hi5 cells were transfected with pIE2-sPgCad1-GFP (a–d) or pIE2-r14PgCad1-GFP (e–h). Cells in (d) and (h) were treated with 10 or 40 μg Cry1Ac per mL, respectively, and observed for swelling using fluorescence microscopy. Arrows in (d) point to representative swollen cells. Cells in (a–c) and (e–g) are untreated controls, all shown at the same magnification. For these controls, GFP-labeled PgCad1 fusion proteins are green and nuclei stained with Hoechst 3342 are blue. Superimposed images from (a–b) are shown in (c), and from (e–f) in (g).
