Figure 1

RT-PCR amplification of marker gene mRNAs in human satellite cells. Electropherograms of amplified products are shown. Fragments of the PAX3, PAX7, Myf5, MyoD, and myogenin genes were amplified from total RNA of satellite cells (S) and skeletal muscle (M). In the satellite cells, expected size product was obtained at different density from PAX3, PAX7, Myf5, and myogenin, but not from MyoD. In contrast, all 5 fragments were amplified in skeletal muscle (M). The expected size of each amplified product is shown on the right margin of each electropherogram. Mk refers to the 100Â bp ladder size marker. Full gel-images are available in Supplementary Figs. 1, 2 and 3.