Figure 3 | Scientific Reports

Figure 3

From: Dystrophin Dp71ab is monoclonally expressed in human satellite cells and enhances proliferation of myoblast cells

Figure 3

Western blot assay of Dp71ab in human satellite cells. Lysates of human satellite cells and AGS cells transfected with Dp71 and Dp71ab-expression plasmids were analyzed by Western blotting using antibodies ab12577 and 7A10, directed against different domains of the C-terminal region of dystrophin. The immunoblotting results are shown. Binding of ab12577 (A) and 7A10 (B) antibodies to proteins in AGS cells expressing Dp71 (Dp71) and Dp71ab (Dp71ab) was disclosed. These antibodies also bound to a protein in human satellite cells (S) located at the same position as artificially expressed Dp71ab. The blots were reacted with antibody to β-actin as a loading control (bottom). The left margins show molecular size markers. Full gel-images are available in Supplementary Figs. 6, 7, and 8.

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