Figure 4
Myoblast cell proliferation. (A) CCK-8 assay of the proliferation of myoblasts transfected with the mock-, Dp71- and Dp71ab- plasmids at 72 h (left). The absorbance of cells expressing Dp71ab was significantly higher than the absorbance of the Dp71- or mock-transfected cells (left). Absorbance is expressed as the mean ± standard error of the mean (SEM) of three independent experiments. Time course showing that cell absorbance began to increase significantly from baseline at 48 h (right). *p < 0.05, **p < 0.01. (B) Time course of numbers of myoblasts transfected with the mock-, Dp71- and Dp71ab-plasmids from 0 to 72 h. Cells were counted in a microscopic field of each well at 0, 24, 48 and 72 h. Data are expressed as mean ± SEM of three independent experiments. **p < 0.01, ***p < 0.001.
